聯(lián)系我們/CONTACT US

聯(lián)系人：錢經理

電話：13636351217

手機：13636351217，13636351073

地址：上海市松江臨港科技城漢橋文化科技園B座

郵編：201615

傳真：021-64881400

郵箱：2881726255@qq.com

阿儀網(wǎng)商鋪：http://www.app17.com/c60514/

手機網(wǎng)站：m.shhyswkj.com

產品分類/PRODUCT CLASS

技術文章

恒遠產品文獻:人腫瘤壞死因子α（TNF-α）ELISA試劑盒引用文獻

點擊次數(shù)：14554 發(fā)布時間：2021/1/14 9:02:43

【文獻標題】 The distinct role of CD73 in the progression of pancreatic cancer

【作者】Liangjing Zhou1，Shengnan Jia1，Yan Chen，et.al

【作者單位】浙江大學（Zhejiang University）

【文獻中引用產品】

人腫瘤壞死因子α（TNF-α）ELISA試劑盒

【關鍵詞】Pancreatic cancer，Progression，CD73，TNFR2，miR-30a-5p

【DOI】doi.org/10.1007/s00109-018-01742-0

【影響因子(IF)】4.14

【出版期刊】 《Journal of Molecular Medicine》

【產品原文引用】

Detection of TNF-α levels in supernatant via ELISA

Supernatant was investigated for levels of TNF-α protein using commercial TNF-α ELISAs (Hengyuan Biotechnology Co., Ltd., Shanghai, China) according to the manufacturer’s instructions.

Western blot

Cells were harvested, ａnd total protein lysates were resolved using 10% sodium dodecyl sulfate–polyacrylamide gels,electroblotted onto a polyvinylidene fluoride membrane,blocked by 5% skim milk, ａnd probed with primary antibodies listed in Table S1. The membrane was then incubated with the secondary antibody ａnd visualized by enhanced chemiluminescence using Kodak X-Omat LS film (Eastman Kodak,Rochester, USA).