【文獻標題】Reduced growth capacity of preimplantation mouse embryos in chronic unpredictable stress model
【作者】Xiaoli Zhao，Ruihong Ma，Xiaoyu Zhang，et.al
【作者單位】天津中醫藥大學第附屬醫院（ First Teaching Hospital of Tianjin University of Traditional Chinese Medicine）
【文獻中引用產品】
小鼠促腎上腺皮質激素（ACTH）ELISA試劑盒
小鼠皮質酮/腎上腺酮（CORT）ELISA試劑盒
【關鍵詞】chronic unpredictable stress model, differential gene expression, hypothalamus‐pituitary‐adrenal axis, preimplantation embryos, RNA sequencing
【DOI】DOI: 10.1002/mrd.23439
【影響因子(IF)】2.82
【出版期刊】《Molecular Reproduction ａnd Development》
【產品原文引用】
4.4 | Measurement of serum HPA axis‐associated
hormones
Blood was collected from 20 mice in each group by retro‐orbital bleeding. For serum preparation, the blood samples were left undisturbed at room temperature for 30 min, ａnd then subject to centrifugation at 1000g for 15 min at 4°C. The supernatants were transferred into new tubes ａnd stored at ?80°C until analysis. Serum levels of CRH, ACTH, and CORT were measured by ELISA according to manufacturer＇s manual (H‐20509, H‐20423, H‐20381, respectively; Shanghai Hengyuan Biotechnology Inc.).
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